| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Chlorobium limicola | TGCCGTAGATGCCATGCT | TTCGGGAAGCCTTGCGTT | 59.09 | 59.89 | 268 | 89 |
100.00%
|
100.00%
|
| Primer 302 | Chlorobium limicola | AGCTTGCCGTAGATGCCA | TTCGGGAAGCCTTGCGTT | 59.01 | 59.89 | 272 | 89 |
100.00%
|
100.00%
|
| Primer 303 | Chlorobium limicola | TGCCATGCTGACCGAACAT | TTCGGGAAGCCTTGCGTT | 60.00 | 59.89 | 259 | 89 |
100.00%
|
100.00%
|
| Primer 304 | Chlorobium limicola | ATGCCATGCTGACCGAACA | TTCGGGAAGCCTTGCGTT | 60.00 | 59.89 | 260 | 89 |
100.00%
|
100.00%
|
| Primer 305 | Chlorobium limicola | CGGCAGAAACTCCCGAACA | ACGGCAAGTTCGGCAGTT | 60.30 | 60.20 | 283 | 89 |
100.00%
|
100.00%
|
| Primer 306 | Chlorobium limicola | TGGCATCTTCACGCTGCA | AGCGGAAATCGGGTGTCA | 59.97 | 58.93 | 153 | 88 |
100.00%
|
100.00%
|
| Primer 307 | Chlorobium limicola | AACAGCTGCGGTGACTCA | CCTCTGTTGCTGCTGTTGC | 59.18 | 59.71 | 218 | 88 |
100.00%
|
100.00%
|
| Primer 308 | Chlorobium limicola | AACAGCTGCGGTGACTCA | AGGACCTCTGTTGCTGCTG | 59.18 | 59.63 | 222 | 88 |
100.00%
|
100.00%
|
| Primer 309 | Chlorobium limicola | ACAGCGTTGCCGTCATCA | TCGCGTTCGCATAGCACA | 59.97 | 60.13 | 193 | 88 |
100.00%
|
100.00%
|
| Primer 310 | Chlorobium limicola | AACAAGGGAGCCAGGAACG | AACCACAAACGCCTGGGA | 59.93 | 59.40 | 165 | 88 |
100.00%
|
100.00%
|